The aim of this present work was to develop and validate a simple, rapid, accurate RP-HPLC method for the estimation of dolutegravir sodium in bulk. Mobile phase consisted of acetonitrile and HPLC water in the ratio of 1:1% v/v and was run through a column of Agilent TC-C18 (2), 5 µm, 4.5×250 nm at a flow rate of 1.5 ml/min and the eluents were monitored at 250 nm. The percent recoveries ranged between 95-105 % and RSD < 2%. Linearity was observed in the concentration range between 20 to 100% with correlation coefficient >0.995¬¬¬¬. The percent of relative standard deviation of six replicate measurements was found to be 0.972 which indicates that the method was precise. The proposed RP-HPLC method was validated according to ICH guidelines and was successfully employed for routine quality control analysis in bulk.
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